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The Matlacha Pass National Wildlife Refuge is part of the United States National Wildlife Refuge System, located within the Matlacha Pass estuary, approximately 8 miles northwest of Fort Myers, Florida. The 538 acre (2.2 km2) refuge was established on September 26, 1908. The islands were established as a "preserve and breeding ground for native birds" by President Theodore Roosevelt. It is administered as part of the J. N. "Ding" Darling National Wildlife Refuge Complex.

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